Academic Knowledge Archives of Gunma Institutes >
群馬大学(Gunma University) >
35 医学部 >
3503 群馬大学医療技術短期大学部 >
350301 群馬大学医療技術短期大学部紀要 >
第01巻 (1980) >

Please use this identifier to cite or link to this item: http://hdl.handle.net/10087/1800

Title: Effect of Temperature on Polyacrylamide Gel Electrophoresis and Chromatography of Histones and Protamines
Authors: Hamana, Koei
Issue Date: 10-Mar-1981
Publisher: 群馬大学医療技術短期大学部
Citation: 群馬大学医療技術短期大学部紀要. 1, 106-119 (1981)
Abstract: Polyacrylamide gel electrophoresis or chromatography of histories (H1, H5, H2A, H2B, H3 and H4) and protamines (salmine and clupeine) in 0.9M acetic acid or 0.01M HC1, respectively, was carried out at various temperatures. A retardation of either electrophoretic migration or chromatographic elution of the proteins was observed at lower temperatures. H1 and H5 histones exhibited the least extent of retardation. The retardation with lowering temperature was also observed in the presence of 0.02-0.05M NaCl but not in the presence of 5-6.25M urea. The extent of retardation in polyacrylamide gel chromatography with lowering temperature increased with proteins of higher arginine contents. The fact was explained as that ionic interactions between guanido groups of arginine residues in the proteins and carboxyl groups of polyacrylamide are strengthened at lower gel temperatures.
URI: http://hdl.handle.net/10087/1800
ISSN: 0389-7540
Appears in Collections:第01巻 (1980)

Files in This Item:

File Description SizeFormat
KJ00004436648.pdf596.93 kBAdobe PDFView/Open

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.


DSpace Software Copyright © 2002-2010  Duraspace - Feedback