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第03巻 (1982) >

Please use this identifier to cite or link to this item: http://hdl.handle.net/10087/1816

Title: Effect of the Degree of Cross-Linkage of Polyacrylamide Gel on the Electrophoretic Mobility of Histones
Authors: Hamana Koei
Issue Date: 30-Mar-1983
Publisher: 群馬大学医療技術短期大学部
Citation: 群馬大学医療技術短期大学部紀要. 3, 169-178 (1983)
Abstract: The electrophoretic mobility in polyacrylamide gel of calf thymus histones was examined as a function of the degree of cross-linkage (C,%) of the gel in five different buffer systems. With increasing the degree of cross-linkage, the mobility once decreased and then increased, irrespective of total gel concentrations (T,%) used (5-15%). The degree of cross-linkage of the gel where the mobilities of the histones became minimum (C_<min>), 7-15%C, was larger than that where the mobilities of other high molecular weight proteins become minimum, 5-7%C. The retardation coefficients (K_R) of the histones obtained from changes of the total gel concentration attained their maximum values in gels which have C_<min>. In gels of C_<min> an approximately linear relationship was obtained between the K_R and the molecular weight of the histones.
URI: http://hdl.handle.net/10087/1816
ISSN: 0389-7540
Appears in Collections:第03巻 (1982)

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