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第04巻 (1983) >

Please use this identifier to cite or link to this item: http://hdl.handle.net/10087/1826

Title: Triton-Gel Electrophoresis of Histones Containing Oxidized Methionine and Cysteine Residues
Authors: Hamana, Koei
Issue Date: 30-Mar-1984
Publisher: 群馬大学医療技術短期大学部
Citation: 群馬大学医療技術短期大学部紀要. 4, 115-127 (1984)
Abstract: Electrophoresis in polyacrylamide gels in the presence of 6mM Triton X-100 in 0.9M acetic acid and 6.25M urea gave a high resolution of separation of the histones in which methionines and cysteines, respectively, are oxidized to the sulfoxides or sulfones (H2A, H2B, H3, H4) and to form an intramolecular disulfide bond (H3). The methionine-oxidized histones migrated faster than untreated histones. The electrophoretic mobility of histone H3 also increased by oxidation of the two cysteines to a cystine. The increase of the mobilities might be explained in terms of the decrease of the helical contents of the histones by the oxidation of methionine and/or cysteine residues.
URI: http://hdl.handle.net/10087/1826
ISSN: 0389-7540
Appears in Collections:第04巻 (1983)

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