Academic Knowledge Archives of Gunma Institutes >
群馬大学(Gunma University) >
35 医学部 >
3503 群馬大学医療技術短期大学部 >
350301 群馬大学医療技術短期大学部紀要 >
第02巻 (1981) >

Please use this identifier to cite or link to this item: http://hdl.handle.net/10087/1863

Title: Gel Electrophoresis, Gel Chromatography and Sedimentation Analysis of Histones and Protamines in the Presence of Nonionic Surfactants
Authors: Hamana, Koei
Issue Date: 10-Mar-1982
Publisher: 群馬大学医療技術短期大学部
Citation: 群馬大学医療技術短期大学部紀要. 2, 165-177 (1982)
Abstract: Histones and protamines behaved as a monomeric form in 0.9M acetic acid. When gel electrophoresis was carried out in 0.9M acetic acid, the mobilities of calf H2A, H2B, H3 and H4 histones were decreased proportionaly to the helix content of the histones but the mobilities of calf H1 histone and fish protamines were not decreased by the addition of 6mM nonionic surfactant into the gel. Degree of the decreasing on the mobilities of four histones was also proportional to the molecular weight of the added nonionic surfactant. Structure of hydrophobic group of the surfactants was more affective than polyoxyethylene chain length of the surfactants. H2B, H3 and H4 histones aggregated in 0.05-0.1M sodium phosphate buffer, pH 7.0, while H1 and H2A histones behaved as a monomeric form. Nonionic surfactants bound to H2A histone but did not to H1 histone and to the aggregates of other histones in the buffer.
URI: http://hdl.handle.net/10087/1863
ISSN: 0389-7540
Appears in Collections:第02巻 (1981)

Files in This Item:

File Description SizeFormat
KJ00004436675.pdf1.05 MBAdobe PDFView/Open

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.


DSpace Software Copyright © 2002-2010  Duraspace - Feedback