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第09巻 (1988) >

Please use this identifier to cite or link to this item: http://hdl.handle.net/10087/1956

Title: SDS-Polyacrylamid Gel電気泳動法による尿タンパク分画の検討
Other Titles: Urinary Protein Fraction Shown by SDS-Polyacrylamid Gel Electrophoresis
Authors: 原, 文子
小山, 景子
町田, 哲男
福村, 幸仁
牛島, 義雄
Keywords: Urinary Protein Fraction
SDS-PAGE
Silver Staining
Issue Date: 31-Mar-1989
Publisher: 群馬大学医療技術短期大学部
Citation: 群馬大学医療技術短期大学部紀要. 9, 47-53 (1989)
Abstract: To study urinary proteins electrophoretically, a highly sensitive silver staining method was applied to urinary protein fractions separated by SDS-polyacrylamide gel electrophoresis. With this method, urine specimens from normal human subjects containing about 10mg of protein/dl could be separated into 25 protein bands without any pretreatment. Proteins having molecular weights from 230,000 to 17,000 were included in these bands. Five protein bands with molecular weights of 170,000, 74,000, 68,000 49,000 and 40,000 were present in all of the 4 urine specimens from normal human subjects. The protein bands with molecular weights of 170,000, 68,000 were identified by the immunoblotting technique as IgG and albumin, respectively.
URI: http://hdl.handle.net/10087/1956
ISSN: 0389-7540
Appears in Collections:第09巻 (1988)

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