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第12巻 (1991) >

Please use this identifier to cite or link to this item: http://hdl.handle.net/10087/1983

Title: ABC-ELISA-A new method suitable for large-scale serological assessment of malaria
Authors: Sato, Kumiko
Yamaguchi, Haruyasu
Kano, Shigeyuki
Suzuki, Mamoru
Keywords: ABC-ELISA
ELISA
Malaria serological test
Issue Date: 31-Mar-1992
Publisher: 群馬大学医療技術短期大学部
Citation: 群馬大学医療技術短期大学部紀要. 12, 133-141 (1992)
Abstract: The authors developed a new practical method for the measurement of malaria antibodies which met the requirements needed for large-scale malaria assessment in the tropics. The new method, ABC-ELISA has used the avidin biotin peroxidase complex system for the amplification of the ordinary ELISA substrate reactions in malaria serological tests. The ABC-ELISA was more sensitive than the ordinary ELISA through the use of the ABC system. This allowed the resulting color spots on the dried plate wells to be read clearly with the naked eye. The method was able to differentiate well malaria patients with recent past history from subjects. The sera from malaria patients were examined using three kinds of sero-immunological methods: ABC-ELISA, fluorescence enzyme linked immunosorbent assay (fluorescence-ELISA), and indirect fluorescent antibody technique (IFAT). An excellent correlation between the ABC-ELISA and IFAT titers was obtained; a correlation coefficient being 0.923. ABC-ELISA titers also correlated well with fluorescence-ELISA titers; the correlation coefficient being 0.962. The lyophilization of the malaria antigen preserved the antigen activity without inducing any nonspecific reaction. These results suggest that the ABC-ELISA is useful for assessing malaria survey in tropical endemic areas, because the test can be carried out without using major electrical equipment.
URI: http://hdl.handle.net/10087/1983
ISSN: 0389-7540
Appears in Collections:第12巻 (1991)

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