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Please use this identifier to cite or link to this item: http://hdl.handle.net/10087/2958

Title: Gamma Irradiation Effects on Cultured Cells: Investigated by the MTT Method
Authors: Bunnak, Jintana
Takigami, Machiko
Ito, Hitoshi
Shinozawa, Takao
Keywords: Gamma-irradiation
Caski cell line
MTT method
Counting method
Mitochondria
Issue Date: Dec-1994
Publisher: 日本放射線影響学会
Citation: Journal of radiation research. 35(4), 205-212 (1994)
Abstract: Effects of gamma-irradiation, given in the range of 5 to 30 Gy on Caski cells (Epitheloid carcinoma from the cervix) were investigated by the MIT (3-(4,5-dimethylthiazol-2-yl) -2,5-diphenyl tetrazolium bromide) method. Results were compared with data assessed simultaneously from cell number counts. The sizes of cells irradiated with 10 to 30 Gy were larger than those of unirradiated ones, and each irradiated cell reduced a larger amount of MT[ than did each unirradiated cell. Irradiation in the above range, therefore causes Caski cells to lose their ability to divide, but the effect on the mitochondria was very slight. Application of the MIT method to the irradiated cells should be done with care. Because, in the irradiated cells depending on the irradiation dose, the MTT activity does not correlate to the cell number.
URI: http://hdl.handle.net/10087/2958
ISSN: 0449-3060
Appears in Collections:学術雑誌論文

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