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330501 The Kitakanto medical journal >
Vol.56 (2006) >

Please use this identifier to cite or link to this item: http://hdl.handle.net/10087/3075

Title: The Stimulatory Effect of Activin on PRL Production in GH3 Cells in a Serum-Free Culture
Authors: Ibuki, Yuji
Abe, Yumiko
Ibuki, Yoshito
Minegishi, Takashi
Keywords: Activin
Follistatin
Prolactin
GH3 cells
Issue Date: 1-Feb-2006
Publisher: 北関東医学会
Citation: The Kitakanto Medical Journal. 56(1), 1-9 (2006)
Abstract: Background and Aims: Activin was originally found as a stimulating factor of follicle stimulating hormone in pituitary cell cultures. To establish a simple culture system for studying the effects of activin on prolactin production, we examined the effects of activin in rat somatolactotroph derived GH3 cells cultured in a serum free condition. Methods: GH3 cells were cultured in Ham’s F10 medium containing 2 mM L-glutamine and 0.1% (w/v) BSA with activin, follistatin (an activin binding protein), or a vehicle (control). Results: The cell viability was between 80 to 90% throughout the 96 hour incubation period in the three groups. Activin stimulated prolactin production in a time dependent (24-96 hours)and dose dependent (75-300 ng/ml)manner. A co-treatment with graded doses of follistatin and 100 ng/ml of activin suppressed the increase of prolactin production brought about by activin in a dose dependent manner, and the production decreased to the control level after the addition of 300 ng/ml of follistatin. Conclusions: The present study demonstrated the stimulatory effect of activin on prolactin production and the antagonism between activin and follistatin in GH3 cells in a serum free condition. This culture system is thus useful to study the effects of activin on prolactin production.
URI: http://hdl.handle.net/10087/3075
ISSN: 1343-2826
Appears in Collections:Vol.56 (2006)

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