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330501 The Kitakanto medical journal >
Vol.58 (2008) >

Please use this identifier to cite or link to this item: http://hdl.handle.net/10087/3476

Title: Impaired Proliferation and Th1 Differentiation of CD4+ T Cells of SHPS-1 Mutant Mice
Authors: Kaneko, Yuka
Kaneko, Yoriaki
Ohnishi, Hiroshi
Tomizawa, Takeshi
Okajo, Jun
Saito, Yasuyuki
Okuzawa, Chie
Murata, Yoji
Okazawa, Hideki
Nojima, Yoshihisa
Okamoto, Koichi
Matozaki, Takashi
Keywords: Th1/Th2 cells
autoimmunity
cell surface molecules
transgenic/knockout mice
Issue Date: 1-May-2008
Publisher: 北関東医学会
Citation: The Kitakanto medical journal. 58(2), 133-139 (2008)
Abstract: Background & Aims: SHPS-1 is a transmembrane protein that binds the protein tyrosine phosphatases SHP-1 and SHP-2 through its cytoplasmic region. It is highly expressed on the surface of CD11c+ dendritic cells (DCs) and macrophages. We have recently shown that priming of CD4+T cells by DCs is markedly impaired in mice that express a mutant form of SHPS-1 lacking most of the cytoplasmic region. We have now evaluated further the functions of CD4+T cells derived from SHPS-1 mutant mice. Methods: The expression of cell surface molecules on CD4+T cells was examined by flow cytometry. The proliferation of CD4+T cells was measured by[3H]thymidine incorporation. Cytokine production by CD4+T cells was measured by ELISA. Results: SHPS-1 is expressed at low level on CD4+T cells of wild-type mice. The T cell receptor (TCR)-stimulated proliferation of CD4+T cells from SHPS-1 mutant mice was markedly decreased, whereas the TCR-stimulated production of IL-2 and IFN-γ by these cells was markedly increased, compared with those apparent with wild-type cells. Differentiation of CD4+T cells from SHPS-1 mutant mice into Th1 cells was also impaired. Conclusions: Present results suggest that SHPS-1 is essential for proper regulation of CD4+T cell functions.
URI: http://hdl.handle.net/10087/3476
ISSN: 1343-2826
Appears in Collections:Vol.58 (2008)

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