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Please use this identifier to cite or link to this item: http://hdl.handle.net/10087/9641

Title: 蛍光性低酸素誘導因子の相関分析
Other Titles: Fluorescence correlation analysis of GFP-labelled HIF-1α in stable
Authors: 田中, 悠樹
野村, 保友
タナカ, ユウキ
ノムラ, ヤストモ
Tanaka, Yuki
Nomura, Yasutomo
Keywords: 低酸素誘導因子
Fluorescence correlation spectroscopy
Brownian motion
Diffusion equation
Diffusion coefficient
Issue Date: 10-Nov-2015
Abstract: HIF-1α, hypoxia induced factor-1αis regarded as a target for drug development in several diseases such as cancer. For high throughput screening of HIF-1α-targeted drug, we need to determine the activity sensitively and quantitatively. In the present study, we proposed a method of fluorescence correlation analysis on HIF-1α activation in which Co2+ treatment against stable transformants of GFP-labelled HIF-1α mimicked hypoxia. In fluorescence correlation spectroscopy, we observed fluorescence intensity fluctuation within a volume element which fluorescence molecules enter and exit because of the Brownian motion in solution or cytosol. When one-component model was used for the analysis, it was difficult to discriminate diffusion coefficient of active form of HIF-1α with inactive one. In two-component model, however, a fraction of slow moving component, GFP-labelled HIF-1α increased significantly when the transformants were exposed to Co2+. In the case of high throughput screening for HIF-1α-targeted drug with fluorescence correlation spectroscopy, we should use the fraction of the slower moving component to judge the activation.
URI: http://hdl.handle.net/10087/9641
Appears in Collections:第18号(2015)

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