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No.26(2015) >

Please use this identifier to cite or link to this item: http://hdl.handle.net/10087/9873

Title: 膵臓リパーゼ活性の阻害に及ぼすキトサンの影響
Other Titles: The Effect of Chitosan on the Inhibition of Pancreatic Lipase Activity
Authors: 榮, 昭博
関﨑, 悦子
Sakae, Akihiro
Sekizaki, Etsuko
Keywords: リパーゼ活性阻害
lipase activity inhibition
bile acid
Issue Date: 20-Dec-2015
Publisher: 桐生大学紀要委員会
Citation: 桐生大学紀要 26, 51-56 (2015)
Abstract: 食物繊維が膵臓リパーゼ活性に及ぼす影響を明らかにするため,胆汁酸で乳化したオリーブ油を基質とする酵素反応にキトサン,アルギン酸ナトリウム,コンドロイチン硫酸,ペクチンを添加してリパーゼ活性阻害を調べた. また,食物繊維の添加が基質の乳化状態に及ぼす影響を明らかにするため,胆汁酸で乳化した脂肪乳濁液(乳化脂肪)にキトサン,アルギン酸ナトリウム,コンドロイチン硫酸,ペクチン添加して,①油層状態の変化,②乳化状態の変化(解乳化),③これらの食物繊維と胆汁酸の吸着率を比較した.その結果,次のことが明らかになった. 1. キトサンはリパーゼ活性を阻害した.アルギン酸ナトリウム,コンドロイチン硫酸,ペクチンは,リパーゼ活性を阻害しなかった. 2. キトサン,アルギン酸ナトリウム,コンドロイチン硫酸,ペクチンを乳化脂肪に加えると,全ての条件において短時間で水層と油状層に分離した.しかし食物繊維添加 24時間後の油層の状態はキトサンと他の食物繊維では異なっていた.また,乳化状態もキトサンを添加した場合には大きな粒子が観察された. 3. キトサンの胆汁酸吸着率は 60% で他の食物繊維は 1~2% であった.  以上のことから,キトサンによるリパーゼ活性の阻害はキトサンが胆汁酸を吸着し基質の乳化状態が変化させたことにより生じたことが示唆された. To investigate the effects of dietary fiber on the pancreatic lipase activity, chitosan, sodium alginate, chondroitin sulfate and pectin were added to the enzyme reaction using olive oil as substrate, and the inhibitory activity was examined.  Furthermore, to investigate the effects of dietary fiber addition on the emulsified state of the substrate, chitosan, sodium alginate, chondroitin sulfate and pectin were added to the substrate emulsified by bile acid. We have observed the following three points: ① changes in the oil layer state, ② change of emulsified state (demulsification) , and ③ adsorption rate of bile acid in the case of addition of these dietary fiber. As a result, the following results were drawn: 1. Lipase activity was inhibited by the addition of chitosan. The addition of sodium alginate, chondroitin sulfate and pectin did not inhibit lipase activity.  2. The addition of dietary fiber to the substrate caused a separation into an aqueous layer and an oily layer in a short time in all conditions. However, the substrate state 24 hours later after the fiber addition differed in chitosan and other dietary fibers. In addition, in the case of the emulsion state after adding the chitosan, large particles were observed.  3. The bile acid adsorption rate was 60% for bile acids and it was 1‒2% for the other dietary fibers.  Our results suggest that the inhibition of lipase activity by chitosan is due to the changes in the emulsified state of substrate by the adsorption of bile acids.
URI: http://hdl.handle.net/10087/9873
ISSN: 13424076
Appears in Collections:No.26(2015)

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